KIAA0202, a Human Septin Family Member, Interacting with hPFTAIRE1

YANG Tao, GAO Yan-Kun, CHEN Jiang-Ye^*
( State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstract    hPFTAIRE1 is a member of Cdc2-related kinase family localized in cytoplasm. To search its substrates and regulatory proteins, the hPFTAIRE1 was fused to LexA and used as bait to screen a human brain LexA two-hybrid library. In this screening, 7 hPFTAIRE1 interacting proteins, including KIAA0202, were obtained. The interaction between the KIAA0202 and the hPFTAIRE1 was confirmed by using immunoprecipitation. The KIAA0202 is a 508 aa protein and is a member of septin family. The central portion of the KIAA0202 is a GTP_-CDC domain, which is conserved among almost all septins. Its C-terminus is a coiled-coil structure and its N-terminus is a variable region. Our results suggest that the KIAA0202 may play important roles in functional regulation of hPFTAIRE1.
Key words    hPFTAIRE1； KIAA0202； septin； yeast two-hybrid

^*Corresponding author：Tel: 86-21-64374430-5251; Fax, 86-21-64338357; e-mail, [email protected]c.cn